DNA methylation was measured using the PyroMark MD system (Qiagen) as described previously.12 (link) Oligonucleotide primers for the nearby miR-137 CpG island published previously16 (link) were purchased from Thermo Fisher Scientific. Primer sequences for the distal CpG island of miR-137 in the P1 and P2 regions are given in Supplementary Table 2 . Methylation was quantified using the software provided (Qiagen).
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Methylation Quantification of miR-137 CpG Islands
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Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College
Other organizations : Binzhou University, Binzhou Medical University, The University of Texas Health Science Center at San Antonio
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Variable analysis
independent variables
- DNA methylation measurement using the PyroMark MD system (Qiagen)
- DNA methylation levels
- Oligonucleotide primers for the nearby miR-137 CpG island published previously
- Primer sequences for the distal CpG island of miR-137 in the P1 and P2 regions
- No positive or negative controls were explicitly mentioned in the input.
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