At baseline, a 10-ml spot urine sample was collected for urinalysis which detects proteinuria by using Sysmex UF-1000i analyzer (TOA Medical Electronics, Kobe, Japan). Fasting blood samples were drawn for laboratory examination.
Serum creatinine and cystatin C were determined using Roche Cobas 8000 modular analyzer Series (Roche, Inc., Mannheim, Germany) with the enzymatic assay creatinine Plus Ver.2 (Roche, Mannheim, Germany) and a particle-enhanced immunonephelometric assay (Roche, Tina-quant Cystatin C, Mannheim, Germany) on cobas c702 and cobas c501 platforms, respectively.
We calculated GFR by Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations. We chose the combined creatinine–cystatin C equation of CKD-EPI which performed better than only creatinine or only cystatin C equation as the primary GFRcrcys estimation method [13 (link)].
Serum creatinine and cystatin C were determined using Roche Cobas 8000 modular analyzer Series (Roche, Inc., Mannheim, Germany) with the enzymatic assay creatinine Plus Ver.2 (Roche, Mannheim, Germany) and a particle-enhanced immunonephelometric assay (Roche, Tina-quant Cystatin C, Mannheim, Germany) on cobas c702 and cobas c501 platforms, respectively.
We calculated GFR by Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations. We chose the combined creatinine–cystatin C equation of CKD-EPI which performed better than only creatinine or only cystatin C equation as the primary GFRcrcys estimation method [13 (link)].
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