The Agilent human lncRNA + mRNA Array V4.0 was used for the profiling of lncRNA and mRNA in MCF-7, LCC2, and LCC9 cell lines. Each array contains probes that could interrogate around 41,000 lncRNAs and 34,000 mRNAs in human genome. The target sequences for these lncRNA and mRNA were derived from a wide range of databases: 23,898 from GENCODE/ENSEMBL, 21,488 from LNCipedia, 14,353 from Human LincRNA Catalog (13 (link)), 13,701 from NRED (ncRNA Expression Database), 7,760 from RefSeq, 5,627 from USCS, 3,019 from LncRNAs-a (Enhancer-like), 1,053 from Antisense ncRNA pipeline, 1,038 from H-InvDB, 962 UCRs, 848 from Chen Ruisheng lab (Institute of Biophysics, Chinese Academy of Science), and 407 Hox ncRNAs.
The CapitalBiotech human circRNA Array V2.0 was used for the profiling of circRNA in MCF-7, LCC2, and LCC9 cell lines. Each array contains probes capable of interrogating approximately 170,340 human circRNAs, the target sequences of which are from Circbase, Deepbase and publication of Rybak-Wolf (14 (link)). Each circRNA was simultaneously detected by a long probe and a short probe.
The CapitalBiotech human circRNA Array V2.0 was used for the profiling of circRNA in MCF-7, LCC2, and LCC9 cell lines. Each array contains probes capable of interrogating approximately 170,340 human circRNAs, the target sequences of which are from Circbase, Deepbase and publication of Rybak-Wolf (14 (link)). Each circRNA was simultaneously detected by a long probe and a short probe.
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