GUS histochemical location was conducted as previously described [1 (link)]. Each photograph in Fig. 2 f is representative of at least nine tissues from three replicated experiments. Fluorometric quantitative analysis was measured according to Jefferson’s method [16 (link)]. The control or transgenic samples were analysed to determine GUS activities with the substrate of 1 mM 4-methyl umbelliferyl β-d -glucuronide (Sigma-Aldrich, Shanghai, China). Fluorescence values were recorded with a Hitachi 850 Fluorescence spectrophotometer (Hitachi, Tokyo, Japan). The protein concentration was determined as described by Bradford [17 (link)].
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