We measured hs-cTnT on stored blood samples collected during Visit 4 (1996–1998) with a high-sensitivity assay, Elecsys Troponin T (Roche Diagnostics®), on an automated Cobas e411 analyzer.25 (link) We used hs-cTnT cutoffs based on prior studies, with the limit of detection of 5 ng/L.25 (link),42 (link), Similarly, we also measured NT-proBNP on the automated Cobas e411 analyzer (Roche Diagnostics) using an electrochemiluminescent immunoassay with a measurement range of 5–35,000 pg/mL and a limit of quantification of 35 pg/mL.26 (link)
Protocol detail
Quantifying Cardiac Biomarkers in Blood Samples
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Corresponding Organization : University of Missouri–Kansas City
Other organizations : George Washington University, Milken Institute, Emory University, Johns Hopkins University, Johns Hopkins Medicine, Mayo Clinic, Baylor College of Medicine, Houston Methodist, Michael E. DeBakey VA Medical Center, University of North Carolina at Chapel Hill, University of Mississippi Medical Center
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Variable analysis
independent variables
- None explicitly mentioned
- Hs-cTnT (high-sensitivity cardiac troponin T)
- NT-proBNP (N-terminal pro-B-type natriuretic peptide)
- Stored blood samples collected during Visit 4 (1996–1998)
- Positive control: None mentioned
- Negative control: None mentioned
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