Protocol detail

Tunicamycin-Induced ER Stress Response

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Mice were injected intraperitoneally with 1mg/kg tunicamycin (catalog #T7765; Sigma, St. Louis, Missouri) dissolved in DMSO (vehicle control). tunicamycin is known to cross blood brain barrier [31 (link)] and previous studies have shown that tunicamycin administration (1 mg/kg; IP) induces increases in ER stress markers in mouse brain [32 (link), 33 (link)]. The behavioral effects of ERB-041 are less well characterized than those of other ER subtype agonists, such as propyl pyrazole triol (PPT) and diarylpropionitrile (DPN). The few behavioral studies used 0.9 mg/kg of DPN and PPT; and showed that both drugs enhanced novel object recognition [34 (link)] and object placement [35 (link)]. Given the high affinity and selectivity of ERB-041 for ERβ [36 (link)], we chose 1 mg/kg to use in our study. It is known that ERB-041 reaches maximum brain levels 30 minutes after injection [36 (link)]. Therefore, ERB-041 (catalog #PZ0183; Sigma, St. Louis, Missouri) was administered 30 minutes before tunicamycin injection.

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Crider A., Nelson T., Davis T., Fagan K., Vaibhav K., Luo M., Kamalasanan S., Terry AV J.r, & Pillai A. (2018). Estrogen receptor β agonist attenuates endoplasmic reticulum stress-induced changes in social behavior and brain connectivity in mice. Molecular neurobiology, 55(9), 7606-7618.

Publication 2018

tunicamycin ERB-041

Agonists Blood brain barrier Brain Diarylpropionitrile Dmso Drugs Erb 041 Mouse Propyl pyrazole triol Selectivity Tunicamycin

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Tunicamycin (1 mg/kg, IP)

dependent variables

Behavioral effects of ERB-041

control variables

DMSO (vehicle control)

controls

Positive control: Previous studies have shown that tunicamycin administration (1 mg/kg; IP) induces increases in ER stress markers in mouse brain.
Negative control: Not explicitly mentioned.

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