Antibodies used for immunoblot and immunofluorescence analysis were obtained from the following sources: mouse anti–dynamin 1/2 and mouse anti–dynamin 2 were from BD; rabbit anti–dynamin 2 was described previously (Ferguson et al., 2009 (link)); mouse anti–DC-STAMP, mouse anti-actin, mouse anti–cathepsin K, and rabbit anti–integrin β3 were from EMD Millipore; rabbit anti-Src and mouse anti-NFATc1 were from Santa Cruz Biotechnology, Inc.; mouse anti-vinculin was from Sigma-Aldrich; rabbit anti-CHC was from Abcam; anti–mouse and anti–rabbit IgG-HRP conjugates were from Promega; and Alexa Fluor 488– and 546–conjugated secondary antibodies were from Invitrogen.
Protocol detail
Antibody Sources for Immunoblot and Immunofluorescence
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Actin
Alexa fluor 488
Anti igg
Antibodies
Cathepsin k
Dynamin 2
Immunoblot
Immunofluorescence
Integrin
Mouse
Promega
Rabbit
Vinculin
Corresponding Organization : Harvard University
Other organizations : Yale University, Howard Hughes Medical Institute
Top 5 similar protocols
Variable analysis
independent variables
- Mouse anti–dynamin 1/2
- Mouse anti–dynamin 2
- Rabbit anti–dynamin 2
- Mouse anti–DC-STAMP
- Mouse anti-actin
- Mouse anti–cathepsin K
- Rabbit anti–integrin β3
- Rabbit anti-Src
- Mouse anti-NFATc1
- Mouse anti-vinculin
- Rabbit anti-CHC
- Immunoblot analysis
- Immunofluorescence analysis
- Anti–mouse and anti–rabbit IgG-HRP conjugates
- Alexa Fluor 488– and 546–conjugated secondary antibodies
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!