The experimental model to induce HD was previously described [10 (link)21 (link)]. Briefly, 3-nitropropionic acid (3NP; Sigma, St. Louis, MO, USA) was dissolved in saline (25 mg/mL) and passed through a 0.2-µm filter. We followed the 3NP injection protocol described by Huang et al. [21 (link)], with 3NP injected intraperitoneally twice daily for two days at 12-h intervals (10:00 a.m. and 10:00 p.m.) at a dose of 60 mg/kg for the first two injections and 80 mg/kg for the second two injections.
Protocol detail
Huntington's Disease Induction Protocol
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3 nitropropionic acid
Saline
Corresponding Organization :
Other organizations : Catholic University of Daegu, Dankook University, Catholic University of Korea
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Variable analysis
independent variables
- 3-nitropropionic acid (3NP) dose
- Injection frequency (twice daily for two days)
- Not explicitly mentioned
- Saline solution preparation (25 mg/mL, 0.2-μm filtered)
- Injection route (intraperitoneal)
- Injection timing (12-h intervals)
- Positive control: Not specified
- Negative control: Not specified
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