The E. coli KEIO collection 29 was arrayed on solid agar plates in 1536 format, and plates were incubated at room temperature for 30 hours. Medium used was LB 0% NaCl supplemented with 40 µg/ml Congo red (CR) and 20 µg/ml Coomassie blue.
The S. Typhimurium single gene deletion library 55 (link) was arrayed on solid agar plates in 384 format, and plates were incubated at room temperature for 48 hours. Medium composition was the same as for E. coli.
The P. aeruginosa PA14 single deletion transposon library 30 (link) was arrayed on solid agar plates in 384 format, and plates were incubated at room temperature. Medium used was TB (Tryptone Broth) supplemented with 20 µg/ml CR and 10 µg/ml Coomassie blue.
All plates were imaged under controlled lighting conditions (spImager S&P Robotics Inc.) using a 18 megapixel Canon Rebel T3i (Canon Inc. USA).
The S. Typhimurium single gene deletion library 55 (link) was arrayed on solid agar plates in 384 format, and plates were incubated at room temperature for 48 hours. Medium composition was the same as for E. coli.
The P. aeruginosa PA14 single deletion transposon library 30 (link) was arrayed on solid agar plates in 384 format, and plates were incubated at room temperature. Medium used was TB (Tryptone Broth) supplemented with 20 µg/ml CR and 10 µg/ml Coomassie blue.
All plates were imaged under controlled lighting conditions (spImager S&P Robotics Inc.) using a 18 megapixel Canon Rebel T3i (Canon Inc. USA).
