Blood was sampled at the end of periods 1 and 2 in the morning when measures noted above were made, and samples were also taken after period 3. Samples were collected by jugular venipuncture into two tubes used for plasma, one with sodium fluoride and potassium oxalate and another with sodium heparin, and a third tube without an anticoagulant for serum. Plasma and serum were harvested by centrifugation for 20 min at 3000× g and frozen at −20 °C. Plasma from the sodium fluoride and potassium oxalate tube was analyzed for glucose and lactate, respectively, with a USI 2300 Plus Glucose & Lactate Analyzer (YSI Inc., Yellow Springs, OH, USA). Plasma from the sodium heparin tubes and (or) serum was analyzed for constituents such as non-esterified fatty acids (NEFA), triglycerides, cholesterol, urea, albumin, and total protein with a Vet Axcel® Chemistry Analyzer (Alfa Wassermann Diagnostic Technologies, West Caldwell, NJ, USA) according to the manufacturer’s instructions. Plasma was analyzed for total antioxidant capacity (TAC) colorimetrically with a Technicon Autoanalyzer II System (Technicon Instruments, Tarrytown, NY, USA) based on a ferric reducing ability of plasma [29 (link)].
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