Quantifying Inflammatory Markers in Lung Samples

Caspase-3 activity in lung and cell lysates was assayed using a Caspase-3 Colorimetric Assay Kit (Biovision, Milpitas, CA, USA) according to the manufacturer’s instructions. Myeloperoxidase (MPO) activity was measured in lung lysates and BAL cell pellets using the EnzChek Myeloperoxidase Activity Assay Kit (Life Technologies, Carlsbad, CA, USA). Total RNA was isolated from lung homogenates and cultured macrophages. Total RNA was isolated (Qiagen, Valencia, CA, USA), and specific transcripts were quantified by real-time PCR using TaqMan FAM-labeled probes (Applied Biosystems, Foster City, CA, USA) as described by Rohani et al.27 (link) Total IL-1β levels were measured using the Mouse IL-1β ELISA Ready-SET-Go! Kit (eBioscience).

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Vandivort T.C., Birkland T.P., Domiciano T.P., Mitra S., Kavanagh T.J, & Parks W.C. (2017). Stromelysin-2 (MMP-10) facilitates clearance and moderates inflammation and cell death following lung exposure to long multiwalled carbon nanotubes. International Journal of Nanomedicine, 12, 1019-1031.

Publication 2017

Caspase-3 Colorimetric Assay Kit EnzChek Myeloperoxidase Activity Assay Kit TaqMan FAM-labeled probes

Assay Caspase 3 Cell Colorimetric Elisa Il 1β Lung Macrophages Mouse Myeloperoxidase Pellets Real time pcr

Corresponding Organization :

Other organizations : Cedars-Sinai Medical Center, Lung Institute, New Jersey Institute of Technology, University of Washington

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Caspase-3 activity in lung and cell lysates
Myeloperoxidase (MPO) activity in lung lysates and BAL cell pellets
Expression of specific transcripts quantified by real-time PCR
Total IL-1β levels

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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