HSV-1 Corneal Infection in Mice

Male and female WT and CD118-/- mice (6-10 weeks of age) were anesthetized by i.p. injection with xylazine (6.6 mg/kg) and ketamine (100 mg/kg) followed by scarification of the cornea using a 25 5/8-gauge needle. The tear film was then blotted, and the cornea was topically inoculated with 1,000 PFU of HSV-1 in 3 μl of RPMI-1640 medium. HSV-1 viral titers were determined in the designated tissue at times post infection (pi) by plaque assay as previously described (32 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Conrady C.D., Thapa M., Wuest T, & Carr D.J. (2009). Loss of Mandibular Lymph Node Integrity is Associated with an Increase in Sensitivity to HSV-1 Infection in CD118 Deficient Mice. Journal of immunology (Baltimore, Md. : 1950), 182(6), 3678-3687.

Publication 2009

Assay Cd118 Cornea Female Hsv 1 Infection Ketamine Male Mice Needle Plaque Tear Tissue Xylazine

Corresponding Organization :

Other organizations : Bipar, University of Oklahoma Health Sciences Center

Top 5 similar protocols

Protocol cited in 6 other protocols

Variable analysis

independent variables

Genotype (WT and CD118-/- mice)

dependent variables

HSV-1 viral titers in the designated tissue at times post infection (pi)

control variables

Age of mice (6-10 weeks)
Anesthesia (xylazine and ketamine)
Corneal scarification (using a 25 5/8-gauge needle)
Inoculation (1,000 PFU of HSV-1 in 3 μl of RPMI-1640 medium)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!