Northern blotting was carried out for selected siRNAs according to the method described by Cai et al. (2018) (link). After 10-min denaturation treatment, 20 µg of total RNAs were loaded into a 15% (w/v) TRIS BORATE-EDTA (TBE)–urea polyacrylamide gel and then resolved by electrophoresis at 150 V for ∼1 h. The resulting RNA samples were transferred from the gel to a Hybond NX membrane (Hybond-NX, GE Healthcare, Chicago, IL, USA) using the semi-dry method. The membrane was subjected to 90-min EDC [a1-ethyl-3-(3-dimethylaminopropyl) carbodiimide]-mediated chemical crosslinking at 65°C, and hybridized with biotin-labeled probes, which are complementary to the siRNAs or U6 (Supplemental Table S11), at 55°C overnight. After washes, the membrane was detected with the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher, Waltham, MA, USA) according to the manufacturer’s instructions. Chemiluminescent signals were recorded with CheiScope 3300 Mini (CLINX, China). Three biological repeats were performed for each siRNA.