Quantifying SARS-CoV-2 Viral Load

To measure viral load in participants with COVID-19 and rule out asymptomatic infection in controls, quantitative reverse transcription PCR in all mid-turbinate swabs collected at enrolment using United States Centers for Disease Control and Prevention primers and probes designed for the detection of SARS-CoV-2 was performed [16 (link)]. High viral load was defined as a cycle threshold value for the detection of the coronavirus nucleocapsid gene region 1 below the median of all samples collected at enrolment (day 1) from SARS-CoV-2-infected participants.

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Rosas-Salazar C., Kimura K.S., Shilts M.H., Strickland B.A., Freeman M.H., Wessinger B.C., Gupta V., Brown H.M., Boone H.H., Rajagopala S.V., Turner J.H, & Das S.R. (2023). Upper respiratory tract microbiota dynamics following COVID-19 in adults. Microbial Genomics, 9(2), mgen000957.

Publication 2023

Coronavirus Covid 19 Gene Infection controls Nucleocapsid Primers Reverse transcription Sars cov 2 Turbinate

Corresponding Organization :

Other organizations : Vanderbilt University Medical Center, Vanderbilt University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Viral load in participants with COVID-19
Asymptomatic infection in controls

control variables

Quantitative reverse transcription PCR in all mid-turbinate swabs collected at enrolment
United States Centers for Disease Control and Prevention primers and probes designed for the detection of SARS-CoV-2

controls

Positive control: Participants with COVID-19
Negative control: Asymptomatic participants (controls)

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