ChIP were carried out as described previously28 (link). Briefly, LNCaP cells were cross-linked with 1% formaldehyde for 10 min and the reaction is quenched by 0.125 M glycine for 5 min at RT. Cells were then rinsed with cold 1 × PBS twice, incubated with cell lysis buffer and subsequently nuclear lysis buffer. Chromatin was sonicated and fragmented to a size of 200–500bp, pre-cleared with agarose/protein A or G beads (Upstate), and incubated with 3–5 ug of antibody (anti-FOXA1 from Abcam, cat# ab23738; anti-AR from Millipore, cat#06-680; and anti-GATA2 from Santa Cruz, cat#H-116) overnight and the protein-DNA complexes were then precipitated, washed, and eluted. ChIP-seq library preparation and sequencing were performed as described previously28 (link).