We obtained Ucp1-luciferase mice from the laboratory of Dr. Shingo Kajimura (University of California, San Francisco) [15 (link)]. Briefly, 150 mg/kg of D-Luciferin potassium salt (GoldBio, St. Louis, MO, USA) was injected into WT and Tph1 FKO with Ucp1-luciferase fed SCD or HFD. Fifteen minutes post-injection, we detected luciferase activity using IVIS Lumina S5, using the Living Image software (PerkinElmer, Waltham, MA, USA) to setup (autoexposure), analyze, and organize data.
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In Vivo Bioluminescence Imaging of Ucp1
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Other organizations : Korea Advanced Institute of Science and Technology, Gwangju Institute of Science and Technology, Yonsei University
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Variable analysis
independent variables
- Diet (SCD or HFD)
- Luciferase activity
- Genotype (WT and Tph1 FKO with Ucp1-luciferase)
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