Cytoskeleton Dynamics Modulation by Inhibitors

Inhibition experiments were performed with blebbistatin (Sigma-Aldrich, USA) or with the ROCK inhibitor Y27632 (Sigma-Aldrich, USA) at concentrations as indicated. Cells were fixed with 2% paraformaldehyde (Sigma-Aldrich, USA) in PBS. Actin cytoskeleton was visualized with phalloidin-Alexa Fluor 647 (ThermoFisher, USA). For flow cytometry, integrin β6 was stained as described previously with an antibody developed by the antibody facility at the University of Geneva (Kessler et al., 2022 (link)). Integrin αV was stained with a commercial antibody (ThermoFisher, USA, clone RMV-7, 1:1000 diluted). Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech, USA, 1:1000 diluted). Actin in living cells was visualized with SiR actin (Spirochrome, Switzerland).