EVs from study participants were available from a previous study (18 (link)) and were precipitated by an isolation kit (miRCURY Exosome Isolation Kit, Qiagen, Venlo, Netherlands), characterized by Nanoparticle Tracking Analysis (ZetaView PMX 110, Particle Metrix, Meerbusch, Germany) and visualized by transmission electron microscopy as described earlier (23 (link)).
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