Fresh stool specimens or anal swabs were collected from cases. The best specimens were a fecal specimen, anal swab was used only when the patient had no stool specimen. Specimens collected were tested as soon as possible. Specimens placed in the culture-Blair medium were tested within 24 h of refrigeration. Fresh fecal samples were placed in clean, dry containers without soap or disinfectant residue, and sent for examination within 8 h of refrigeration.
Isolation and identification of Salmonella were performed as described in the Operation Procedure for Salmonella Inspection in the Foodborne Disease Surveillance Work Manual of the National Center for Food Safety Risk Assessment. In brief, the above specimens were placed in SBG augmenting solution and cultured at 36°C for 18 to 24 h. Furthermore, after gently shaking the expanding liquid tube we applied 1 ring line to the Salmonella chromogenic medium or XLD AGAR plate and incubated it at 36 ± 1°C for 18 to 24 h. We picked three to five suspected colonies, inoculated in TSI AGAR, lysine decarboxylase, and nutrient AGAR plates, at 36 ± 1°C for 18 to 24 h. A single colony was scraped from a nutrient AGAR plate for systematic biochemical identification. Either of biochemical identification kit or automatic microbial biochemical identification system can be selected for identification.
The Salmonella serovar was identified with specific O and H antiserum samples according to the Kauffmann–White scheme as described in the instructions provided by the manufacturer of the antiserum samples (Statens Serum Institute, SSI).
Isolation and identification of Salmonella were performed as described in the Operation Procedure for Salmonella Inspection in the Foodborne Disease Surveillance Work Manual of the National Center for Food Safety Risk Assessment. In brief, the above specimens were placed in SBG augmenting solution and cultured at 36°C for 18 to 24 h. Furthermore, after gently shaking the expanding liquid tube we applied 1 ring line to the Salmonella chromogenic medium or XLD AGAR plate and incubated it at 36 ± 1°C for 18 to 24 h. We picked three to five suspected colonies, inoculated in TSI AGAR, lysine decarboxylase, and nutrient AGAR plates, at 36 ± 1°C for 18 to 24 h. A single colony was scraped from a nutrient AGAR plate for systematic biochemical identification. Either of biochemical identification kit or automatic microbial biochemical identification system can be selected for identification.
The Salmonella serovar was identified with specific O and H antiserum samples according to the Kauffmann–White scheme as described in the instructions provided by the manufacturer of the antiserum samples (Statens Serum Institute, SSI).
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