Identification of GPCRs in Diverse Ciliates

To identify the GPCRs of ciliates, we collected the genome data of 24 ciliates, and the dataset was derived from four sources. Of the collected ciliates, the data for Ichthyophthirius multifiliis, Tetrahymena borealis, Tetrahymena empidokyrea, Tetrahymena paravorax, Tetrahymena pyriformis, Tetrahymena shanghaiensis, Tetrahymena thermophila, Euplotes octocarinatus, Euplotes vannus, Stentor coeruleus, Oxytricha trifallax, and Stylonychia lemnae were obtained from Ciliates.org (https://ciliates.org/landing/ (accessed on 12 October 2022)). The data for Entodinium caudatum, Carchesium polypinum, Halteria grandinella, Pseudocohnilembus persalinus, and Spirostomum minus were retrieved from NCBI (www.ncbi.nlm.nih.gov (accessed on 12 October 2022)). The data for Paramecium caudatum, Paramecium sexaurelia, and Parameciium tetraurelia were obtained from ParameciumDB (https://paramecium.i2bc.paris-saclay.fr/ (accessed on 12 October 2022)). Further ciliate data, including data for Epistylis chlorelligerum, Epistylis plicatilis, Vorticella convallara, and Zoothamnium arbuscula can be accessed through the National Genomics Data Center (https://ngdc.cncb.ac.cn/ (accessed on 12 October 2022)). This dataset covers four classes, eight orders, twelve families, and fifteen genera. In addition, we also identified the GPCRs in three non-ciliates species (used as outgroups), including Perkinsus marinus, Symbiodinium microadriaticum, and Plasmodium falciparum. These data were also obtained from NCBI (www.ncbi.nlm.nih.gov (accessed on 17 October 2022)).