Determination of Fe(III) Reduction Activity
Corresponding Organization : University of Córdoba
Other organizations : Universidad Autónoma Agraria Antonio Narro
Variable analysis
- Pre-treatment of plants for 30 min in plastic vessels with 50 mL of a nutrient solution without micronutrients, pH 5.5
- Transfer of plants into 50 mL of a Fe (III) reduction assay solution for 1 h, consisting of nutrient solution without micronutrients, 100μM Fe(III)-EDTA and 300 μM Ferrozine, pH 5.0
- Fe (III) reduction, measured spectrophotometrically by the absorbance (562 nm) of the Fe(II)-Ferrozine complex and using an extinction coefficient of 29.800 M^-1 cm^-1
- Root fresh weight
- SPAD values (as a proxy of the chlorophyll concentration in leaf), measured daily with a portable chlorophyllmeter
- Environmental conditions during the measurement of Fe (III) reduction were the same as the growth conditions described above
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