Focal Demyelination and HMGB1 Treatment

Focal demyelinated lesions were induced by injecting 1 μl of 1.0% LPC (Sigma-Aldrich, MA, USA) in PBS into the spinal cord ventral funiculus of 11-week-old mice as previously described (Psachoulia et al., 2016 (link)). For the treatment group, 1 μl of 1 mg/ml recombinant human HMGB1 protein (Abcam, Cambridge, UK) in water was co-injected along with 1.0% LPC into the ventral spinal cord. For the control group, 1 μl of 1 mg/ml recombinant human HMGB1 protein (Abcam, Cambridge, UK) in water was co-injected with the vehicle, sterile phosphate buffered saline (PBS).

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Rouillard M.E., Hu J., Sutter P.A., Kim H.W., Huang J.K, & Crocker S.J. (2022). The Cellular Senescence Factor Extracellular HMGB1 Directly Inhibits Oligodendrocyte Progenitor Cell Differentiation and Impairs CNS Remyelination. Frontiers in Cellular Neuroscience, 16, 833186.

Publication 2022

recombinant human HMGB1 protein

Hmgb1 Human ml 1 protein Mice Phosphate Saline Spinal cord Sterile

Corresponding Organization : University of Connecticut

Other organizations : Georgetown University

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Variable analysis

independent variables

Injection of 1 μl of 1.0% LPC (Sigma-Aldrich, MA, USA) in PBS into the spinal cord ventral funiculus of 11-week-old mice
Co-injection of 1 μl of 1 mg/ml recombinant human HMGB1 protein (Abcam, Cambridge, UK) in water along with 1.0% LPC into the ventral spinal cord

dependent variables

Focal demyelinated lesions

control variables

Age of mice (11-week-old)
Co-injection of 1 μl of 1 mg/ml recombinant human HMGB1 protein (Abcam, Cambridge, UK) in water with the vehicle, sterile phosphate buffered saline (PBS)

negative control

Co-injection of 1 μl of 1 mg/ml recombinant human HMGB1 protein (Abcam, Cambridge, UK) in water with the vehicle, sterile phosphate buffered saline (PBS)

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