Culturing Endothelial Cells from Human Sources

Human umbilical vein endothelial cells (HUVECs) (Lonza, Mississauga, Ontario, Canada) were cultured as previously described (45 (link)). Human dermal microvascular endothelial cells (HMVECds) from pooled donors (Lonza) and human brain endothelial cells hCMEC/d3 (Millipore) were cultured at 37°C in an atmosphere of 5% CO₂ in endothelial growth medium-2 microvascular (EGM-2 MV) and EndoGro (Millipore, Etobicoke, Ontario, Canada) supplemented with basic fibroblast growth factor (Millipore), respectively.

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Lithgow K.V., Church B., Gomez A., Tsao E., Houston S., Swayne L.A, & Cameron C.E. (2020). Identification of the Neuroinvasive Pathogen Host Target, LamR, as an Endothelial Receptor for the Treponema pallidum Adhesin Tp0751. mSphere, 5(2), e00195-20.

Publication 2020

HUVECs HMVECds hCMEC/d3 EndoGro basic fibroblast growth factor

Atmosphere Basic fibroblast growth factor Brain Cells Donors Endothelial Endothelial cells Growth medium Human Human umbilical vein endothelial cells

Corresponding Organization :

Other organizations : University of Victoria, University of Washington

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Variable analysis

independent variables

Cell type (Human umbilical vein endothelial cells (HUVECs), Human dermal microvascular endothelial cells (HMVECds), and human brain endothelial cells hCMEC/d3)

dependent variables

Not explicitly mentioned

control variables

Culture conditions (37°C in an atmosphere of 5% CO2)
Culture media (endothelial growth medium-2 microvascular (EGM-2 MV) and EndoGro (Millipore, Etobicoke, Ontario, Canada) supplemented with basic fibroblast growth factor (Millipore))

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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