High molecular weight (HMW) DNA from D. kikkawai (adult females), D. takahashii (adult females), and D. bipectinata (adult males and females) flies was provided by Dr. Bernard Kim at Stanford University. An overview of the DNA extraction protocol has previously been described (Kim, Miller, et al. 2021 (link); Kim, Wang, et al. 2021 (link)).
The library preparation and PacBio sequencing were performed by the McDonnell Genome Institute (MGI) at Washington University in St. Louis. The SMRTbell Express Template Prep Kit 2.0, Sequel Binding Kit 3.0, and Sequel Sequencing Kit 3.0 were used to prepare the samples for single-molecule real-time (SMRT) sequencing using the PacBio Sequel system. Each species was sequenced using one 1M SMRT cell in the continuous long-read (CLR) sequencing mode with the 6.0.0.45111 chemistry and a movie length of 600 min. The sequencing data were processed by version 7.0.1.66975 of PacBio SMRT Link.
To assess the quality of the PacBio sequencing data, the quality control (QC) tool in SequelTools (Hufnagel et al. 2020 (link)) and SEQUELstats were used to analyze the subreads and scraps BAM files for each species.
The library preparation and PacBio sequencing were performed by the McDonnell Genome Institute (MGI) at Washington University in St. Louis. The SMRTbell Express Template Prep Kit 2.0, Sequel Binding Kit 3.0, and Sequel Sequencing Kit 3.0 were used to prepare the samples for single-molecule real-time (SMRT) sequencing using the PacBio Sequel system. Each species was sequenced using one 1M SMRT cell in the continuous long-read (CLR) sequencing mode with the 6.0.0.45111 chemistry and a movie length of 600 min. The sequencing data were processed by version 7.0.1.66975 of PacBio SMRT Link.
To assess the quality of the PacBio sequencing data, the quality control (QC) tool in SequelTools (Hufnagel et al. 2020 (link)) and SEQUELstats were used to analyze the subreads and scraps BAM files for each species.
Free full text: Click here
