Extracellular Vesicle Characterization by Immunoblotting

EV isolation and characterization by IB was carried out as reported earlier [47 (link), 48 (link)]. Blocked membranes were probed with either rabbit monoclonal antibodies (mAbs) to TSG101 (ab125011) from Abcam (Cambridge, UK) and CD63 (55051) and FAK (13009) from Cell Signaling Technologies (Danvers, MA, USA) or mouse mAbs to human CD81 (ab23505) from Abcam, Alix (sc53540) and human CD9 (sc13118) from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and human SERPINA1 (NBP2-52557) from Novus Biologicals (Littleton, CO, USA).

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Sayeed A., Dalvano B.E., Kaplan D.E., Viswanathan U., Kulp J., Janneh A.H., Hwang L.Y., Ertel A., Doria C, & Block T. (2020). Profiling the circulating mRNA transcriptome in human liver disease. Oncotarget, 11(23), 2216-2232.

Publication 2020

ab125011 ab23505)

Biologicals Human Isolation Membranes Monoclonal antibodies Mouse Novus Rabbit Serpina1 Tsg101

Corresponding Organization :

Other organizations : Baruch S. Blumberg Institute, University of Pennsylvania, Medical University of South Carolina, The University of Texas Health Science Center at Houston, Sidney Kimmel Cancer Center, Thomas Jefferson University, Capital Health

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Variable analysis

independent variables

Probing blocked membranes with different types of antibodies

dependent variables

Expression levels of proteins (TSG101, CD63, FAK, CD81, Alix, CD9, SERPINA1) on the extracellular vesicles (EVs)

control variables

EV isolation and characterization protocol used as reported in previous studies [47, 48]

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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