Micro-CT Imaging of Liver and Spleen Tumors

Micro-CT scan (Inveon Micro-CT, Siemens, Germany) was performed 1 day after injection of a contrast agent (ExiTron nano 12000, Miltenyi Biotec, Germany) which is an alkaline earth-based nanoparticulate contrast agent for mouse liver CT imaging [23 (link)]. Mice were injected with 100 μl of contrast agent via a lateral tail vein. Since this agent is taken up by cells of the reticuloendothelial system including macrophages within the liver and spleen, normal liver and spleen were enhanced and tumors were drawn as a black spot.

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Sugase T., Lam B.Q., Danielson M., Terai M., Aplin A.E., Gutkind J.S, & Sato T. (2020). Development and optimization of orthotopic liver metastasis xenograft mouse models in uveal melanoma. Journal of Translational Medicine, 18, 208.

Publication 2020

Inveon Micro-CT ExiTron nano 12000

Cells Contrast agent Ct scan Liver Liver macrophages Mice Micro ct Reticuloendothelial system Scan Spleen Tail Tumors Vein

Corresponding Organization :

Other organizations : Sidney Kimmel Cancer Center, Thomas Jefferson University, University of California, San Diego

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Variable analysis

independent variables

Injection of contrast agent (ExiTron nano 12000, Miltenyi Biotec, Germany)

dependent variables

Micro-CT scan of liver and spleen

control variables

Volume of contrast agent injected (100 μl)
Injection site (lateral tail vein)

controls

Positive control: Normal liver and spleen enhanced by contrast agent
Negative control: Tumors drawn as black spots

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