Collection and Preservation of Chinese Jujube Germplasm

In total, 962 Ziziphus accessions were collected from the National Chinese Jujube Germplasm Repository located in Taigu County, Shanxi Province (Taigu) and the National Foundation for Improved Cultivar of Chinese Jujube, Cang County, Hebei Province (Cangzhou) (Table S6). The samples consist of 942 diploid accessions and 15 triploid accessions of Z. jujuba var. jujuba, as well as five accessions of sour jujube (Table S7). All collected fresh young leaves for each accessions were immediately flash frozen in liquid nitrogen and stored at −70 °C until use. Total genomic DNA was extracted from the leaves following described methods4 (link). DNA quality was tested using 1.0% agarose gel electrophoresis, and the DNA was diluted to 10 ng/μL.

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Xu C., Gao J., Du Z., Li D., Wang Z., Li Y, & Pang X. (2016). Identifying the genetic diversity, genetic structure and a core collection of Ziziphus jujuba Mill. var. jujuba accessions using microsatellite markers. Scientific Reports, 6, 31503.

Publication 2016

Agarose gel electrophoresis Chinese Diploid Frozen Genomic Jujube Nitrogen Triploid Ziziphus

Corresponding Organization :

Other organizations : Beijing Forestry University, Shanxi Academy of Agricultural Sciences

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Variable analysis

independent variables

Jujube accessions collected from the National Chinese Jujube Germplasm Repository in Taigu County, Shanxi Province and the National Foundation for Improved Cultivar of Chinese Jujube in Cang County, Hebei Province

dependent variables

Not explicitly mentioned

control variables

All collected fresh young leaves for each accession were immediately flash frozen in liquid nitrogen and stored at −70 °C until use
Total genomic DNA was extracted from the leaves following described methods4
DNA quality was tested using 1.0% agarose gel electrophoresis
The DNA was diluted to 10 ng/μL

controls

No positive or negative controls were explicitly mentioned in the provided information

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