Anti-HLA Antibody Detection and DSA Identification

Anti-HLA antibody testing was performed using the LABScreen Single Antigen Bead assay (One Lambda, West Hills, CA) with pretreatment by 6% EDTA (Sigma-Aldrich, St. Louis, MO) to remove complement interference (11 (link)). A mean fluorescence intensity (MFI) of 2000 was used as the cutoff for clinical relevance (9 ). Antibody screen results were compared with donor HLA typing (HLA-A, B, C, DR, DP, and DQ) to identify DSAs. For donor alleles not represented in the single antigen bead (SAB) panel, DSA is reported based on beads coated with the closest antigen–determined using the HistoCheck program (12 (link)). Testing was performed either prospectively after November 2014, when routine DSA screening was added to our standard pre-transplant workup, or, in cases before routine DSA screen, retrospectively using banked sera when available.

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Cochran H., Slade M., Gao F., Godbole S., Pruitt A., De Togni E., Liu C., Grossman B, & Abboud R. (2024). Pretransplant Desensitization of Donor-Specific Anti-HLA Antibodies with Plasmapheresis and Immunoglobulin Produces Equivalent Outcomes to Patients with No Donor Specific Antibodies in Haploidentical Hematopoietic Cell Transplant. Research Square.

Publication Preprint 2024

LABScreen Single Antigen Bead assay

Corresponding Organization :

Other organizations : Washington University in St. Louis

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Variable analysis

independent variables

Anti-HLA antibody testing
Pretreatment by 6% EDTA to remove complement interference
Cutoff of mean fluorescence intensity (MFI) of 2000 for clinical relevance
Comparison of antibody screen results with donor HLA typing
Use of HistoCheck program to determine the closest antigen for donor alleles not represented in the single antigen bead (SAB) panel
Timing of testing (prospective after November 2014 or retrospective using banked sera)

dependent variables

Presence and levels of anti-HLA antibodies

control variables

LABScreen Single Antigen Bead assay (One Lambda, West Hills, CA)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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