Protein Purification Techniques

All chemicals, biochemical, and enzyme substrates were obtained from sources described elsewhere.^{7 ,8 (link),1 (link),13 (link),14 (link)} Chromatographic resins (DEAE Sepharose fast flow and Phenyl-Sepharose 6 fast flow) and columns (PD-10 Sephadex G-25M, Econo-Column, and Spectra/Chrom LC) were purchased from commercial vendors, as described.⁷ The Amicon stirred cell concentrators and the ultrafiltration membranes (10,000 Da, MW cutoff) were purchased from EMD Millipore Inc. (Billerica, MA). Coomassie Blue R-250 dye for staining sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels was adapted from a literature source.¹⁵

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Medellin B.P., Lancaster E.B., Brown S.D., Rakhade S., Babbitt P.C., Whitman C.P, & Zhang Y.J. (2020). Structural Basis for the Asymmetry of a 4-Oxalocrotonate Tautomerase Trimer. Biochemistry, 59(16), 1592-1603.

Publication 2020

DEAE Sepharose fast flow PD-10 Sephadex G-25M Econo-Column Spectra/Chrom LC Amicon stirred cell concentrators ultrafiltration membranes

Cell Chrom Chromatographic Coomassie blue r 250 Deae Enzyme Gels Membranes Phenyl sepharose Resins Sds page Sephadex g Sepharose Ultrafiltration

Corresponding Organization : The University of Texas at Austin

Other organizations : University of California, San Francisco

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

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control variables

Chemicals, biochemical, and enzyme substrates obtained from sources described elsewhere
Chromatographic resins (DEAE Sepharose fast flow and Phenyl-Sepharose 6 fast flow) and columns (PD-10 Sephadex G-25M, Econo-Column, and Spectra/Chrom LC) purchased from commercial vendors
Amicon stirred cell concentrators and the ultrafiltration membranes (10,000 Da, MW cutoff) purchased from EMD Millipore Inc. (Billerica, MA)
Coomassie Blue R-250 dye for staining sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels adapted from a literature source

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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