MDCK Cell Culture on Low-Adhesion Plate

MDCK
cells were dispersed on a 96-well plate at a U-shaped low protein
adhesion surface (Prime Surface, Sumitomo Bakelite Co. Ltd., Tokyo,
Japan) at a concentration of 3000 cells/well and cultured for 4 days
in DMEM containing 10% FBS at 37 °C under 5% CO₂ conditions.

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Morimoto N., Murata A., Yamamoto Y., Narita F, & Yamamoto M. (2024). Adhesive Sulfabetaine Polymer Hydrogels for the Sandwich Cell Culture. ACS Omega, 9(10), 11942-11949.

Publication 2024

Prime Surface

Corresponding Organization : Shimane University

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Variable analysis

independent variables

Adhesion surface type (Prime Surface, Sumitomo Bakelite Co. Ltd., Tokyo, Japan)

dependent variables

Cell proliferation/growth over 4 days

control variables

Cell concentration (3000 cells/well)
Culture medium (DMEM containing 10% FBS)
Incubation conditions (37 °C, 5% CO2)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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