Caveolin-2 siRNA Transfection in HUVECs

The HUVECs were transiently transfected with SMART pools human Cav-2 siRNA (Dharmacon, USA) using Lipofectamine^® reagent (Invitrogen, USA) according to the manufacturer’s instructions (Jeon et al., 2015 (link)). Green fluorescence protein (GFP) siRNA (Bioneer, Korea) was used as a control. After 48 h of transfection, the cells were used for subsequent experiments.

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Park H., Shin J.A., Lim J., Lee S., Ahn J.H., Kang J.L, & Choi Y.H. (2022). Increased Caveolin-2 Expression in Brain Endothelial Cells Promotes Age-Related Neuroinflammation. Molecules and Cells, 45(12), 950-962.

Publication 2022

Lipofectamine® reagent GFP) siRNA

Cells Green fluorescence protein Human Lipofectamine Sirna Transfection

Corresponding Organization :

Other organizations : Ewha Womans University

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Variable analysis

independent variables

Cav-2 siRNA

dependent variables

Not explicitly mentioned

control variables

GFP siRNA

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