Syn7002 strains were routinely grown in either AD7 or MAD medium, as previously described (Włodarczyk et al., 2020 (link)). All experiments were performed at 30°C and strains were grown in a Fitotron growth chamber (Weiss Technik), under continuous white LED illumination, at either 50 μmol photons·m−2·s−1 and ambient air or 300 μmol photons·m−2·s−1 and 1.5% CO2 (v/v)-enriched air (using a custom-made airtight enclosure).
Growth and performance of the different strains were tested in 15 ml cultures, grown in upright tissue culture flasks (Corning, part #3056), inoculated at a starting OD730 of 0.1, and measured in a 1 cm-light path UV mini1240 spectrophotometer (Shimadzu) using AD7 as blank. Evaporative water loss was estimated by average weight loss across culture vessels and compensated by daily addition of corresponding volume of sterile MQ water. Whole-cell spectra (between 400 and 730 nm) were recorded in a UV2600i spectrophotometer (Shimadzu), as described above.
All cloning steps were performed using Escherichia coli Stellar supercompetent cells (TaKaRa), grown in Luria-Bertani (LB) medium supplemented with the appropriate antibiotics, as indicated.
Growth and performance of the different strains were tested in 15 ml cultures, grown in upright tissue culture flasks (Corning, part #3056), inoculated at a starting OD730 of 0.1, and measured in a 1 cm-light path UV mini1240 spectrophotometer (Shimadzu) using AD7 as blank. Evaporative water loss was estimated by average weight loss across culture vessels and compensated by daily addition of corresponding volume of sterile MQ water. Whole-cell spectra (between 400 and 730 nm) were recorded in a UV2600i spectrophotometer (Shimadzu), as described above.
All cloning steps were performed using Escherichia coli Stellar supercompetent cells (TaKaRa), grown in Luria-Bertani (LB) medium supplemented with the appropriate antibiotics, as indicated.
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