Cell Lines for HIV Research

TZM-bl cells (ARP-8129³² (link)^,33 (link)^,34 (link)^,35 (link)^,36 (link)^,37 (link)) were obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH (contributed by Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc.) Lenti-X 293T cells (632180, Takara Bio Inc.), TZM-bl cells, HEK293T cells (CRL-3216, ATCC) and COS7 cells (0539, Riken BRC Cell Bank) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (08468-16, Nacalai Tesque, Kyoto, Japan), while HeLa S3 cells (CCL-2.2, ATCC) were cultured in DMEM with low glucose (D6046, Sigma-Aldrich, St. Louis, MO, USA), supplied with 10% (v/v) fetal bovine serum (FBS) (173012, Sigma-Aldrich). HeLa CD4⁺ cells (154, NIH AIDS reagent program) were maintained in DMEM supplemented with 10% (v/v) FBS and 2 mg/mL G418 (16512-81, Nacalai Tesque, Kyoto, Japan). MT4 cells (JCRB1216, Japanese Collection of Research Bioresources Cell Bank), C8166-CCR5 cells, and THP-1 cells were cultured in RPMI-1640 (R8758, Sigma-Aldrich) supplemented with 10% (v/v) FBS. For passaging the adherent cells, cells were treated with 2.5 g/L-trypsin/EDTA solution (32777-44, Nacalai Tesque). All the cells were incubated in a humidified incubator at 37°C with 5% CO₂.