Blood and Bone Marrow Analysis

200–500 μl of blood from adult mice were collected in EDTA-coated tubes (Becton Dickinson) and analyzed on a Bayer ADVIA 120 Hematology System by the Clinical Laboratories of Children's Hospital (Boston, MA). Manual leukocyte differential counts of >300 cells per sample were performed on Wright-Giemsa–stained blood smears. Cytocentrifuge preparations of the bone marrow and spleens of adult mice were also stained with Wright-Giemsa for microscopic evaluation.

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Yu C., Cantor A.B., Yang H., Browne C., Wells R.A., Fujiwara Y, & Orkin S.H. (2002). Targeted Deletion of a High-Affinity GATA-binding Site in the GATA-1 Promoter Leads to Selective Loss of the Eosinophil Lineage In Vivo. The Journal of Experimental Medicine, 195(11), 1387-1395.

Publication 2002

Adult Blood Bone marrow Cells Differential leukocyte counts Edta Mice Microscopic

Corresponding Organization :

Other organizations : Howard Hughes Medical Institute, Harvard University, Dana-Farber Cancer Institute

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Protocol cited in 22 other protocols

Variable analysis

independent variables

Blood collection volume (200–500 μl)

dependent variables

Leukocyte differential counts
Microscopic evaluation of bone marrow and spleen

control variables

Adult mice
EDTA-coated tubes (Becton Dickinson)
Bayer ADVIA 120 Hematology System
Wright-Giemsa staining

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