Blebbistatin Treatment Protocol
Corresponding Organization :
Other organizations : Leibniz-Forschungsinstitut für Molekulare Pharmakologie, National Institutes of Health, National Heart Lung and Blood Institute, Max Delbrück Center, Freie Universität Berlin, Charité - Universitätsmedizin Berlin
Variable analysis
- Concentration of (-)-Blebbistatin (50 µM)
- Duration of (-)-Blebbistatin treatment (1 h)
- Cellular response to (-)-Blebbistatin treatment
- DMEM (Dulbecco's Modified Eagle Medium) as the cell culture medium
- DMSO (Dimethyl Sulfoxide) as the solvent for (-)-Blebbistatin
- Serum-containing culture medium used for wash-out (15 min)
- Positive control: Not specified
- Negative control: Not specified
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