Quantitative Elemental Analysis of Urine

The elementomics quantitative analysis was performed with an iCAP Qc inductively coupled plasma mass spectrometry (ICP-MS) instrument (Thermo Scientific) according to our previous report (Silver et al. 2018 (link)). Briefly,

60 microliters

of urine and

150 microliters

nitric acid were mixed for 3 h, and the samples were mixed with water to the volume of

1.8 milliliters

. After centrifugation, the supernatant was subjected to ICP-MS analysis. The limit of detection was defined as three times of the standard deviation (SD) of the obtained concentrations in the blank with 10 replicates. Quality control and blank samples were analyzed with study samples. Because of the amount of sample available, a total of 963 urine samples in the first trimester were analyzed for the inorganic exposome profiling.

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Chen M., Guan Y., Huang R., Duan J., Zhou J., Chen T., Wang X., Xia Y, & London S.J. (2022). Associations between the Maternal Exposome and Metabolome during Pregnancy. Environmental Health Perspectives, 130(3), 037003.

Publication 2022

iCAP Qc

Centrifugation Exposome Mass spectrometry Nitric acid Plasma Silver Urine

Corresponding Organization :

Other organizations : Nanjing Medical University, National Institute of Environmental Health Sciences, National Institutes of Health

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Elemental concentrations in urine samples

control variables

Volume of urine sample (60 microliters)
Volume of nitric acid (150 microliters)
Final volume of sample diluted to 1.8 milliliters
Centrifugation of samples before ICP-MS analysis
Instrument used: iCAP Qc inductively coupled plasma mass spectrometry (ICP-MS)
Limit of detection defined as three times the standard deviation of blank samples with 10 replicates
Quality control and blank samples analyzed along with study samples

controls

Positive control: Not explicitly mentioned
Negative control: Blank samples analyzed with 10 replicates to determine limit of detection

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