Tissue Dissociation: Collagenase and Trypsin

Tissue samples were minced with a scalpel before enzymatic digestion. For enzymatic disaggregation, we initially incubated with 1 mg/mL collagenase IV (Thermo Fisher Scientific, France) for 1 h, 2.5 h, and 24 h. To shorten incubation time, we introduced trypsin. Subsequently, digestion with 1 mg/mL collagenase IV was combined with 0.25% trypsin-EDTA (Sigma Aldrich, MO, USA): 1 h collagenase with 5 min trypsin, 2 h collagenase with 2 min trypsin.

Free full text: Click here

Piwocka O., Musielak M., Ampuła K., Piotrowski I., Adamczyk B., Fundowicz M., Suchorska W.M, & Malicki J. (2024). Navigating challenges: optimising methods for primary cell culture isolation. Cancer Cell International, 24, 28.

Publication 2024

collagenase IV trypsin-EDTA

Corresponding Organization : Poznan University of Medical Sciences

Other organizations : Adam Mickiewicz University in Poznań, Greater Poland Cancer Center

Top 5 similar protocols

Variable analysis

independent variables

Incubation time with collagenase IV (1 h, 2.5 h, 24 h)
Combination of collagenase IV and trypsin-EDTA (1 h collagenase with 5 min trypsin, 2 h collagenase with 2 min trypsin)

dependent variables

Tissue disaggregation or cell dissociation

control variables

Concentration of collagenase IV (1 mg/mL)
Concentration of trypsin-EDTA (0.25%)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!