Male C57BL/6 mice (6–8 weeks old, 20–25 g weight) and male ICR mice (6–8 weeks old, 25–30 g weight) were purchased from the Model Animal Research Center of Nanjing University (Nanjing, China), where all animals were housed under specific pathogen–free (SPF) conditions. The IL37tg mice on a C57BL/6 background were from Cyagen Biosciences Inc. Briefly, human IL-37 cDNA (NM_014439.3) was cloned and then inserted downstream of the CMV promoter in pRP [Exp] vector (VB150824-10008) (20 (link), 24 (link)). Besides, Pdx1cre mice (International Mouse Strain Resource [IMSR] catalog JAX:014647, RRID: IMSR_JAX:014647, The Jackson Laboratory) and Gsdmdfl/fl (Model Animal Research Center of Nanjing University, Nanjing, China) were intercrossed to generate pancreatic GSDMD-specific knockout (Pdx1cre Gsdmdfl/fl, GsdmdΔPan) (8 (link)).
All animal experiments were conducted in accordance with the Principles of Laboratory Animal Care, and were approved by the Experimental Animal Ethics Committee of Jinling Hospital, affiliated with Nanjing University.
All animal experiments were conducted in accordance with the Principles of Laboratory Animal Care, and were approved by the Experimental Animal Ethics Committee of Jinling Hospital, affiliated with Nanjing University.
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