Total protein was isolated from flash frozen liver tissue as previously described.21 (link) Specific antibodies against FASN (Cell Signaling C20G5), SREBP1 (Novus NB600-582), ADH (Abcam ab108203), CYP2E1 (Abcam Ab19140), ALDH1/2 (SantaCruz sc-166362) were used to quantify protein expression.