Platelet-Rich Plasma Biomarker Analysis

The white blood cell, platelet, lymphocyte, neutrophil, monocyte, and red blood cell counts of the PRP and plasma were measured using a blood analyzer (Advia 2120, Bayer); as described in our previous study22 (link), several growth factors were detected using an enzyme-linked immunosorbent assay (ELISA). The standard curve was obtained for each experiment. Platelet-derived growth factor (PDGF) AA, PDGF BB (RayBiotech Co., Georgia, USA), epidermal growth factor (EGF), and vascular epidermal growth factor (VEGF; Koma Co., Republic of Korea) levels were quantitatively measured using ELISA kits, with reference range was from 0 to 3000 (PDGF AA), from 0 to 2000 (PDGF BB), from 0 to 1000 (EGF) and from 0 to 1000 (VEGF) pg/ml, respectively. All measurements were conducted in triplicate.

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Huang S.H., Wu S.H., Lee S.S., Lin Y.N., Chai C.Y., Lai C.S, & Wang H.M. (2018). Platelet-Rich Plasma Injection in Burn Scar Areas Alleviates Neuropathic Scar Pain. International Journal of Medical Sciences, 15(3), 238-247.

Publication 2018

Advia 2120 PDGF BB

Blood Enzyme linked immunosorbent assay Epidermal growth factor Growth factors Lymphocyte Monocyte Neutrophil Plasma Platelet Platelet derived growth factor aa Platelet derived growth factor bb Red blood cell counts Vascular Vegf White blood cell

Corresponding Organization :

Other organizations : Kaohsiung Medical University, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung Municipal Hsiao-Kang Hospital, National Chung Hsing University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

White blood cell count
Platelet count
Lymphocyte count
Neutrophil count
Monocyte count
Red blood cell count
Platelet-derived growth factor (PDGF) AA level
Platelet-derived growth factor (PDGF) BB level
Epidermal growth factor (EGF) level
Vascular epidermal growth factor (VEGF) level

control variables

None explicitly mentioned

controls

Standard curve obtained for each experiment

Annotations

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