Immunofluorescence Staining of mCherry and TH

These procedures were carried as described previously [28 (link)]. Primary antibodies used were rat monoclonal mCherry (1:2000, Thermo Fisher, M11217); mouse monoclonal TH (1:2000, Sigma, T2928); secondary antibodies were Alexa Fluor 488 goat anti-mouse (1:1000, Invitrogen Molecular Probes, A11001), Alexa Fluor 594 goat anti-rat (1:1000, Invitrogen Molecular Probes, A11007). Slices were mounted on slides, embedded in Mowiol (with 4,6-diamidino-2-phenylindole), cover-slipped, and analyzed using an upright fluorescent microscope (Nikon Eclipse 80i, Nikon).

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Yu X., Ba W., Zhao G., Ma Y., Harding E.C., Yin L., Wang D., Li H., Zhang P., Shi Y., Yustos R., Vyssotski A.L., Dong H., Franks N.P, & Wisden W. (2020). Dysfunction of ventral tegmental area GABA neurons causes mania-like behavior. Molecular Psychiatry, 26(9), 5213-5228.

Publication 2020

Alexa Fluor 488 goat anti-mouse Alexa Fluor 594 goat anti-rat Nikon Eclipse 80i

Alexa 594 Alexa fluor 488 Antibodies Goat Microscope Molecular probes Mouse

Corresponding Organization :

Other organizations : Imperial College London, Xijing Hospital, SIB Swiss Institute of Bioinformatics, University of Zurich, ETH Zurich, UK Dementia Research Institute

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Variable analysis

independent variables

Primary antibodies used: rat monoclonal mCherry (1:2000, Thermo Fisher, M11217); mouse monoclonal TH (1:2000, Sigma, T2928)

dependent variables

Fluorescent signals observed using an upright fluorescent microscope (Nikon Eclipse 80i, Nikon)

control variables

Secondary antibodies used: Alexa Fluor 488 goat anti-mouse (1:1000, Invitrogen Molecular Probes, A11001), Alexa Fluor 594 goat anti-rat (1:1000, Invitrogen Molecular Probes, A11007)
Slices were mounted on slides, embedded in Mowiol (with 4,6-diamidino-2-phenylindole), cover-slipped

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