Bone Marrow Cell Characterization

Bone marrow cells were harvested from mouse femurs and tibias. Goat polyclonal Alp primary antibody (2.5 μg per 10⁶ cells, AF2910, R&D Systems), rabbit polyclonal osteocalcin antibody (1: 10, FL-100, Santa Cruz Biotechnology) and rabbit polyclonal Oscar antibody (1: 10, H-94, Santa Cruz Biotechnology) were used for FACS. Briefly, after washing with PBS containing 1% BSA, the bone marrow cells were incubated with the antibody to Alp or osteocalcin or Oscar before being stained with donkey anti-goat FITC-IgG (1: 200, ab6881, Abcam) or donkey anti-rabbit FITC-IgG (1: 100, sc-2090, Santa Cruz Biotechnology)^{43 (link),44 (link),48 (link)}. The obtained cell populations were washed three times and used for quantitative analysis of chalcone derivative formulations and determination of Smurf1 activity, p-Smad1 and osteocalcin mRNA.

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Liang C., Peng S., Li J., Lu J., Guan D., Jiang F., Lu C., Li F., He X., Zhu H., Au D.W., Yang D., Zhang B.T., Lu A, & Zhang G. (2018). Inhibition of osteoblastic Smurf1 promotes bone formation in mouse models of distinctive age-related osteoporosis. Nature Communications, 9, 3428.

Publication 2018

AF2910 ab6881 sc-2090

Anti igg Antibody Bone marrow cells Cell Chalcone Donkey Femurs Fitc Goat Mouse Mrna Osteocalcin Populations Rabbit Tibias

Corresponding Organization :

Other organizations : Hong Kong Baptist University, Chinese University of Hong Kong, City University of Hong Kong

Top 5 similar protocols

Variable analysis

independent variables

Chalcone derivative formulations

dependent variables

Quantitative analysis of chalcone derivative formulations
Smurf1 activity
P-Smad1
Osteocalcin mRNA

control variables

Bone marrow cells harvested from mouse femurs and tibias
Goat polyclonal Alp primary antibody (2.5 μg per 10^6 cells, AF2910, R&D Systems)
Rabbit polyclonal osteocalcin antibody (1: 10, FL-100, Santa Cruz Biotechnology)
Rabbit polyclonal Oscar antibody (1: 10, H-94, Santa Cruz Biotechnology)
Donkey anti-goat FITC-IgG (1: 200, ab6881, Abcam)
Donkey anti-rabbit FITC-IgG (1: 100, sc-2090, Santa Cruz Biotechnology)

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