Early-life Pharmacological Modulation of the Serotonergic and Oxytocinergic Systems

(R)-(+)-8-hydroxy-2-(di-n-propylamino)tetralin hydrobromide (8OH-DPAT; Sigma, USA), a specific 5-HT_1A receptor full agonist, L-368,899 hydrochloride (Tocris, UK), an OXT receptor antagonist, or OXT (Sigma, USA) was dissolved in saline and subcutaneously injected to pups (8OH-DPAT, 0.5 mg/kg; L-368,899, 3 mg/kg; OXT 83 I.U. [0.2–0.26 mg]/kg) every day from PD7 to PD21.
The treatment doses of 8OH-DPAT^{35 (link)}, L-368,899^{36 (link),37 (link)} and OXT^{30 (link)} were determined based on previous reports. The treatment duration of each drug was determined based on our previous reports^{5 (link)}. After weaning at PD21, the mice were divided into groups of two to five mice per cage. Saline was used as a control.

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Nagano M., Takumi T, & Suzuki H. (2018). Critical roles of serotonin-oxytocin interaction during the neonatal period in social behavior in 15q dup mice with autistic traits. Scientific Reports, 8, 13675.

Publication 2018

8OH-DPAT L-368,899 hydrochloride L-368,899

Drug L 368 899 Mice Saline Tetralin

Corresponding Organization :

Other organizations : Nippon Medical School, RIKEN Center for Brain Science, Japan Science and Technology Agency

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Variable analysis

independent variables

8OH-DPAT (0.5 mg/kg)
L-368,899 (3 mg/kg)
OXT (83 I.U. [0.2–0.26 mg]/kg)

dependent variables

Not explicitly mentioned

control variables

Saline (control)

controls

Saline (negative control)

Annotations

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