Fluorescence Microtiter Plate Spectroscopy

Fluorescence microtiter plate spectroscopy of intact leaf disks was done as previously described (Pasin et al., 2014 (link)). Fifty microliters of water were placed in each well of a Costar flat-bottom black opaque 96 well plate (Corning Inc., Corning, NY, USA) and spread to cover the bottom of the well. Single leaf disks taken with a size 3 cork borer (6 mm diameter) were placed with the adaxial side down on top of the water in the wells. Fluorescence detection was performed using a Synergy 2 reader with Gen5 version 1.10 software (BioTek Instruments Inc., Winooski, VT, USA) and the following read parameters: excitation 485/20, emission 516/50, optics position top 50%, and sensitivity 120.

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MacDonald J., Miletic S., Gaildry T., Chin-Fatt A, & Menassa R. (2017). Co-expression with the Type 3 Secretion Chaperone CesT from Enterohemorrhagic E. coli Increases Accumulation of Recombinant Tir in Plant Chloroplasts. Frontiers in Plant Science, 8, 283.

Publication 2017

Synergy 2 reader Gen5 version 1.10 software

Fluorescence Fluorescence spectroscopy Leaf Optics Sensitivity

Corresponding Organization : Agriculture and Agri-Food Canada

Other organizations : Western University, Université de Bordeaux

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Variable analysis

independent variables

Leaf disks taken with a size 3 cork borer (6 mm diameter)

dependent variables

Fluorescence detection

control variables

Water placed in each well of a Costar flat-bottom black opaque 96 well plate (Corning Inc., Corning, NY, USA) and spread to cover the bottom of the well
Leaf disks placed with the adaxial side down on top of the water in the wells
Fluorescence detection performed using a Synergy 2 reader with Gen5 version 1.10 software (BioTek Instruments Inc., Winooski, VT, USA) with the following read parameters: excitation 485/20, emission 516/50, optics position top 50%, and sensitivity 120

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