Total RNAs from testicular tissues were extracted using an RNeasy Plus Micro Kit (74034, Qiagen, Duesseldorf, Germany) following the manufacturer’s protocol. cDNA was reverse transcribed using a PrimeScript RT reagent Kit (Vazyme, Nanjing, China) and then processed through a SYBR Green-based StepOne-Plus real-time PCR system (Applied Biosystems, Foster City, CA, USA) with 18S rRNA as a normalization control. Primers targeting Espn and 18S rRNA have been previously listed (26 (link)).
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Quantification of Espn Expression in Testicular Tissues
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Corresponding Organization : Nantong University
Other organizations : Nanjing Maternity and Child Health Care Hospital, Soochow University, Affiliated Hospital of Nantong University
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Variable analysis
independent variables
- Extraction method: RNeasy Plus Micro Kit (74034, Qiagen, Duesseldorf, Germany)
- Expression of Espn gene
- 18S rRNA as a normalization control
- Positive control: None specified
- Negative control: None specified
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