Platelets Spreading on Immobilized Substrates

Immunofluorescence studies were performed following the procedure described [19 (link)]. In case of inhibitors treatment, platelets were incubated in the presence or absence of inhibitors and then allowed to spread on immobilized BSA or Fg for 45 min and processed as described [18 (link)]. Stained samples were analyzed using a Zeiss LSM 510 laser-scanning microscope (Thornwood, NY). Differential interference contrast microscopy (DIC) images of the platelets on coverslips were captured using Zeiss Axioskop II light microscope using Zeiss Plan-Apochromat 100x/1.4NA oil immersion lens. The images were digitally recorded using Zeiss AxioVision software as described previously [20 (link)]. Images were processed using Adobe Photoshop Software.