Microbial Analysis of Sonication Fluids and Urine

All sonication fluids and urine samples (1–100) were inoculated into a set of solid media: Blood Agar with 7% sheep’s blood (Oxoid, UK), Endo Agar (Imuna Pharm, Šarišské Michaľany, Slovakia), Wilkins Chalgren Anaerobic Agar with 7% sheep’s blood and vitamin K (WCHA, Oxoid, Hampshire, UK). UCs, DJCs and urine samples were inoculated in addition into Blood Agar with 10% of NaCl, Blood Agar with Amikacine (32 mg/L) (Oxoid, Hampshire, UK), and UriSelect 4 (Bio-Rad, Hercules, CA, USA). Plates were assessed for microbial growth after cultivating at 37 °C for 48 h. The WCHA was cultivated in an anaerobic atmosphere (80% N₂, 10% CO₂, and 10% H₂; Anaerobic Work Station Concept 400, Ruskinn Technology, Bridgend, UK) at 37 °C for 7 days. All isolated strains were identified biochemically or using MALDI-TOF MS (Biotyper with FlexControl 3.4 software, Bruker Daltonics, Billerica, MA, USA), according to the manufacturer’s instructions. For details see [30 (link)].