Plasma Alarin and Adipsin levels were examined using the Human Alarin, Adipsin ELISA Kit (Sunred Biological Technology, Shanghai, China) in a plate-washing -incubation CombiWash device (Human Diagnostics, Wiesbaden, Germany) in accordance with the study procedures determined in the kit catalogue, and the absorbance measurement was taken with a Chromate 4300 Microplate Reader (Awareness Technology, Palm City, USA).
Aqueous analyzes were performed according to a previously published methods [15 (link)]. Two Aqueous liquids and blood samples were enriched with increasing amounts of Adipsin or Alarin. The percentage recovery was calculated as follows: recovered value/expected value × 100.
The measurement range human alarin kit was 5 to 1500 pg/mL and the sensitivity was determined by the manufacturer at 4.638 pg/mL. The intra-assay and inter-assay coefficients of variation for alarin were < 10% and < 12%, respectively. The measurement range of the human Adipsin kit 0.5 to 100 ng/mL and the sensitivity was the determined by the manufacturer at 0.472 ng/mL. The intra-assay and inter-assay coefficients of variation for Alarin were < 10% and < 12%, respectively.
Aqueous analyzes were performed according to a previously published methods [15 (link)]. Two Aqueous liquids and blood samples were enriched with increasing amounts of Adipsin or Alarin. The percentage recovery was calculated as follows: recovered value/expected value × 100.
The measurement range human alarin kit was 5 to 1500 pg/mL and the sensitivity was determined by the manufacturer at 4.638 pg/mL. The intra-assay and inter-assay coefficients of variation for alarin were < 10% and < 12%, respectively. The measurement range of the human Adipsin kit 0.5 to 100 ng/mL and the sensitivity was the determined by the manufacturer at 0.472 ng/mL. The intra-assay and inter-assay coefficients of variation for Alarin were < 10% and < 12%, respectively.
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