All immunoblot analyses were performed as previously described [59 (link)]. Antibodies were obtained from the following sources: antibodies to phospho-T389-S6K1, phospho-S473-AKT, phospho-S235/236-S6, phospho-S65-4E-BP1, 4E-BP1 S6K1, AKT, S6, GAPDH, RICTOR, RAPTOR, DEPTOR, PRAS40, mLST8, mSIN1 were obtained from Cell Signaling Technology. Antibodies to myc (9E10) and FLAG (M2) were obtained from Sigma.
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