Kjeldahl Protein Analysis of Barley Malt

The total amount of protein in barley malt was determined using the Kjeldahl method using the Kjeltec 8100 analyzer (Foss, Copenhagen, Denmark) [19 (link),20 (link)]. This method consists in the mineralization of the sample in a medium of concentrated sulfuric acid (VI) in the presence of catalysts. Protein nitrogen was converted under these conditions to the ammonium ion which, after alkalinization, was distilled in the form of ammonia. The ammonia was determined by acid-base titration with 0.01 MHCl. The standard PN-EN-ISO 20483: 2014-02 was used for marking—Cereals and pulses—Determination of the nitrogen content and calculation of the crude protein content—Kjeldahl method.

Free full text: Click here

Samociuk B., Medyński D., Nowak D., Kawa-Rygielska J., Świechowski K., Gasiński A, & Janus A. (2022). The Use of Barley Malt as a Binder in Molding Sand Technology. Materials, 15(9), 3375.

Publication 2022

Kjeltec 8100

Acid Ammonia Ammonium ion Barley Cereals Foss Mineralization Nitrogen Protein Pulses Sulfuric acid Titration

Corresponding Organization : Witelon State University of Applied Sciences in Legnica

Other organizations : Wrocław University of Science and Technology, AGH University of Krakow, Wroclaw University of Environmental and Life Sciences

Top 5 similar protocols

Variable analysis

independent variables

Kjeldahl method using the Kjeltec 8100 analyzer (Foss, Copenhagen, Denmark)

dependent variables

Total amount of protein in barley malt

control variables

Mineralization of the sample in a medium of concentrated sulfuric acid (VI) in the presence of catalysts
Conversion of protein nitrogen to the ammonium ion under these conditions
Alkalinization and distillation of the ammonia
Determination of the ammonia by acid-base titration with 0.01 MHCl
Use of the standard PN-EN-ISO 20483: 2014-02 for marking—Cereals and pulses—Determination of the nitrogen content and calculation of the crude protein content—Kjeldahl method

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!